Detection of Bordetella avium by TaqMan real-time PCR in tracheal swabs from wildlife birds
نویسندگان
چکیده
منابع مشابه
Detection and differentiation of Bordetella spp. by real-time PCR.
Molecular detection of Bordetella pertussis DNA is a sensitive and specific method for the rapid diagnosis of pertussis. In this study, a new molecular assay for the detection and differentiation of Bordetella spp. based on automated DNA extraction and real-time PCR was evaluated. The analytical sensitivity of the new assay was determined by Probit analysis of serial dilutions of both cloned PC...
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Background and Objective: Sexually infections transmitted by bacteria are one of thetherapeutic and social problemsworldwide. The Real-time PCR assay is one of the most sensitive diagnostic and screening methods for these infections. The purpose of this study wassimultaneous detection of Chlamydia trachomatis and Mycoplasma genitaliumusing the TaqMan duplex real-time polymerase chain reaction. ...
متن کاملDetection of group B streptococci (GBS) in vaginal swabs using real-time PCR with TaqMan probe hybridization.
BACKGROUND & OBJECTIVES Implementation of a screening based strategy for the prevention of perinatal group B streptococcus (GBS) disease is anticipated to increase the demand of fast laboratory techniques. The aim of the present study was to develop a real-time PCR method for the specific detection of GBS in vaginal swabs. METHODS Based on partial sequencing of the sip gene, primers and a Taq...
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Forest fire in recent years has paid great attention to climate change and ecosystems. Remote sensing is a quick and inexpensive way to detect and monitor forest fires on a large scale. The purpose of this study was to identify forest and rangeland fire hazards using NOAA / AVHRR in Kayamaki Wildlife Refuge. For the purpose of this study, the history of the fire-burns occurred in MODIS products...
متن کاملmultiplex real-time pcr assay for detection and differentiation of bordetella pertussis and bordetella parapertussis.
background and objective: rapid diagnosis of pertussis is important for the timely isolation of the infection source and early prevention measures among the contact persons, especially among non-vaccinated infants for whom pertussis is life- threatening. materials and methods: targets is481 , is1001, bp0026 and human gapdh gene were used to develop a multiplex real- time pcr assay based on the ...
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ژورنال
عنوان ژورنال: Polish Journal of Veterinary Sciences
سال: 2017
ISSN: 2300-2557
DOI: 10.1515/pjvs-2017-0005